This document is somewhat inspired by Stephen Diehl's What I Wish I Knew When Learning Haskell and Evan Chen's An Infinitely Large Napkin.
The central dogma of biology (coined by Francis Crick) says that information flows in one direction from DNA to RNA to proteins. Pieces of DNA called exons are transcribed into RNA. RNA is molecularly the same as DNA, except for thiamine which becomes uracil in RNA. RNA is also single, as opposed to double, stranded. Instead of the 4 nucleic acids, proteins in the human body are made of 20 different types of amino acids. A typical protein is 300-400 amino acids long. Three letters of RNA encode an amino acid. This is called translation As there are 4 possible RNA nucleotides, there are possible triplets. More combinations than amino acids. 3 triplets are stop codons, which indicate the end of a protein.
The human genome contains 3,200,000,000 or base pairs, distributed among 46 chromosomes, plus mitochondrial DNA. There are 23,000 protein-coding genes. Unrelated human genomes differ by ~0.1%. Many of the differences are in the form of isolated base substitutions, or single-nucleotide polymorphisms (SNPs). Other differences include small deletions or variations in the length of repetitive regions, including variations in the number of copy genes.
Your genotype is your DNA sequence, both nuclear and mitochondrial. Plants also have chloroplast DNA. Your phenotype is the collection of observable traits other than your DNA sequence. Pharmacogenetics is the pursuit of personalised drugs based on DNA sequences for the prevention and treatment of disease. Your life history is the integrated total of your physical and psychological experiences.
The cells in your body except for gametes (sperm and egg cells), erythrocytes (red blood cells), and cells of the immune system have the same DNA sequence. Epigenetics is the study of how your behaviour and environment can cause changes that affect the way your genes work, without altering your DNA sequence.
| Feature | Prokaryotic Cell | Eukaryotic Cell |
|---|---|---|
| Size | 10m | 100m |
| Subcellular Division | No nucleus | Nucleus |
| State of major component of genetic material | Circular loop, a few proteins are permanently attached | Complexed with histones to form chromosomes |
| Internal differentiation | No organised subcellular structure | Nuclei, mitochondria, chloroplasts, cytoskeleton, endoplasmic reticulum, Golgi apparatus |
| Cell division | Fission | Mitosis (or meiosis) |
Mitochondria and chloroplasts are subcellular particles involved in energy transduction. The former carries out oxidative phosphorylation, the conversion or reducing power from metabolising food into adenosine triphosphate (ATP). The latter carries out photosynthesis, the capture of light energy in the form of nicotinamide adenine dinucleotide phosphate (NADPH) and ATP, leading to the synthesis of sugars.
The nucleic acids are adenine, guanine, cytosine, thymine, and uracil. A and G have two rings and are called purines. C, T, and U have one ring and are called pyrimidines. As always bind to Ts and and Cs always bind to Gs. This lets us tell from one strand of DNA what will be on the other and also enables the DNA to copy itself.
DNA has a direction based on the biochemical properties at each end, the 5 prime end and the 3 prime end. We write one strand 5' to 3' and call that the positive strand. The reverse complement is written 3' to 5' and called the negative strand.
To make a copy of DNA we attach a primer to each end. We separate the strands by warming them up to 94 degrees. When the DNA cools down to 54 degrees, the separated strands stick to their respective primers. At 72 degrees we then use the copier molecule DNA polymerase which looks for sites where the DNA is double-stranded and then goes along the single-stranded gap filling it in. You also need to add raw nucleic acids for the polymerase to use. We can then repeat the cycle, doubling the amount of DNA each time. This is known as a polymerase chain reaction (PRC)
First Generation Sequencing was developed by Frederick Sanger. It is also known as chain termination sequencing.
Next Generation Sequencing, also known as massively parallel sequencing came into use after the Human Genome Project. It works by chopping up DNA into single-stranded fragments attached to a slide. PCR is then used to make clusters of identical fragments on the slide. Nucleotides are then added so that they bind with a single base in each fragment of DNA. They are modified to fluoresce in different colours so we can take pictures to see which base is present and also to have a terminator molecule so we only add one at a time. It is not perfect as errors increase in later cycles which is why it is used on short strands of DNA.
RNA chops up DNA into exons and introns. Introns get thrown away while exons are concatenated together and translated into proteins. Exons make up your exome. It is only ~1.5% of your genome and therefore much more practical to sequence. A protein coding region contains Open Reading Frames (ORF). An ORF is a region of DNA sequence or reasonable length, that begins with an initiation codon. An ORF is a potential protein coding region. Genes in prokaryotes are smaller and do not have introns.
Since RNA is single stranded it can't be sequenced. However, we can transcribe it into complementary DNA (cDNA) with a molecule called reverse transcriptase
An early step in analysing a newly sequenced genome is identifying the genes that code for proteins and RNAs and identifying their products. There are two basic approaches:
A priori methods seek to recognise sequence patterns within expressed genes and the regions flanking them. Protein coding regions have distinctive patterns of codon statistics such as the absence of stop codons.
"Been there, seen that" methods recognise regions corresponding to previously known genes from the similarity of their translated amino acid sequences to known proteins in other species, or by matching Expressed Sequence Tags (EST). ESTs are sequences that correspond to at least part of a transcribed gene.
Haplotypes, or haploid genotypes are local combinations of genetic polymorphisms that tend to be co-inherited. They are a cheap way to characterise genomes.
Mutations in the same DNA molecule in diploid chromosomes will become unlinked by recombination events that occur between their loci. The greater the separation between two sites, the greater the frequency of recombination. Recombination rates vary widely along the genome, by several orders of magnitude. SNPs on opposite sides of recombinational hotspots are more likely to be separated in any generation. SNPs in recombination rare coldspots will tend to stay together.
In humans, many 100-kb regions tend to remain intact. They show the expected number of genomes but relatively few of the possible combinations. An average SNP density of 0.1%, or 1 SNP/kb, suggests ~100 SNPs per 100kb. The genome of any individual may possess or lack each of them giving possible combinations. However, many 100-kb regions have fewer than 5 combinations of SNPs. These discrete combinations of SNPs in recombination-poor regions define an individual's haplotype.
Sequencing the genome of a species for the first time is hard because the data comes to us in fragments and we have to reference to guide us as we try to stitch it together. The fragments are typically about 200 bp long. High-throughput sequencing produces partial sequence information from both these fragments either from one end or both ends. The number of bases reported is the read length. We want to make our read length as big as possible. We then need to assemble the fragments into a continuous stretch of sequence. These partial assemblies are called contigs. We need enough fragments to cover the entire genome and then more so that we can detect errors. The ratio of the total number of bases sequenced to the genome's actual length is called the coverage. Complete and accurate results require a coverage of 30 to 50.
This process is generally enough for prokaryotic genomes. Eukaryotic genomes are more complicated. They need to be broken into smaller pieces and built back up with a genetic map. It's very computationally expensive.
Once a reference genome is published. Future sequences can be mapped onto it. This is straightforward except for highly repetitive regions. Coverage must be adequate so that the error rate of sequence determination is less than natural variation.
For the purpose of compression, DNA bases are often stored as just two bits. Specifically:
| A | 00 |
| C | 01 |
| G | 10 |
| T | 11 |
More modern formats make use of the probability certain bases appear at certain locations.
Used in the 1980s by ... In FASTA format sequences of nucleotides are represented by single letters. The first line of a FASTA file begins with a > or less commonly a ;. Below is the first 5 lines of an example FASTA file.
>NM_002299.4 Homo sapiens lactase (LCT), mRNA
GAAAATGGAGCTGTCTTGGCATGTAGTCTTTATTGCCCTGCTAAGTTTTTCATGCTGGGG
GTCAGACTGGGAGTCTGATAGAAATTTCATTTCCACCGCTGGTCCTCTAACCAATGACTT
GCTGCACAACCTGAGTGGTCTCCTGGGAGACCAGAGTTCTAACTTTGTAGCAGGGGACAA
AGACATGTATGTTTGTCACCAGCCACTGCCCACTTTCCTGCCAGAATACTTCAGCAGTCT
An extension of FASTA. Used in Next Generation Sequencing. It generally produces bigger files which can create headaches when transferring data.
After receiving data from the sequencer, you will normally use a tool such as Burrows-Wheeler Aligner (BWA) to align your sequences to a reference genome. Most users will have a reference genome for their species.
The most common representation for aligned data is the sequence alignment map (SAM) format. The compressed version of SAM is BAM. Indexable for extremely fast random access e.g. to find alignments to a certain part of a chromosome. You need an index for your BAM file which is normally created by the tabix utility of SAMtools, the most widely used tool for manipulating SAM/BAM files.
Logarithmic representation of the probability of an accurate call. This probability is given as . So a Q of 10 represents a 90% call accuracy, 20 represents 99% call accuracy, and 30 will be 99.9%. For our file, the maximum accuracy will be 99.99% (40). In some cases, values of 60 are possible (99.9999% accuracy).
Gets the bases from the opposite strand of DNA
def reverseComplement(s):
complement = {'A': 'T', 'C': 'G', 'G': 'C', 'T': 'A', 'N': 'N'}
t = ''
for base in s:
t = complement[base] + t
return t
A slow but simple way of solving the read alignment problem
def naive(p, t):
occurrences = []
for i in range(len(t) - len(p) + 1): # loop over alignments
match = True
for j in range(len(p)): # loop over characters
if t[i+j] != p[j]: # compare characters
match = False
break
if match:
occurrences.append(i) # all chars matched; record
return occurrences
Illumina make the most popular machines for reading DNA methylation.
Tags: Biology